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PLGA-dendron nanoparticles enhance immunogenicity but not lethal antibody production of a DNA vaccine against anthrax in mice by Suzie Ribeiro; Sjoerd G. Rijpkema; Zarmina Durrani; Alexander T. Florence is a Pharmacology, Toxicology and Pharmaceutics article available to read on EtoBox.

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Dendriplexes, complexes of dendrons and condensed plasmids containing the gene for protective antigen (PA) of Bacillus anthracis, were encapsulated in poly-lactide-co-glycolide (PLGA) particles using the double emulsion method. The two dendrons employed are a dendron with three C 18 chains (C 18 dendron) and one with no attached hydrocarbon chains (the C 0 dendron). Three types of particles were examined, namely PLGA-C 18 dendriplexes, PLGA-C 0 dendriplexes and the control PLGA-naked DNA system. These were characterised by standard biophysical methods such as photon correlation spectroscopy (PCS) and scanning electron microscopy to select the complexes for in vivo testing. Three intramuscular immunizations were carried out using 14 g of DNA per dose at weekly intervals in BALB/c mice. Antibodies against rPA were measured using ELISA. Results indicate that the PLGA-C 18 dendriplex particles produced superior levels of anti-PA IgG antibodies in comparison to animals immunized with the PLGA-C 0 dendriplex particles. The level of antibody production was dependent on the number of immunizations, higher antibody levels being measured after two booster vaccinations. However toxin neutrali

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Author

Suzie Ribeiro; Sjoerd G. Rijpkema; Zarmina Durrani; Alexander T. Florence

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-5173)

Published

2007

Language

EN

Field

Pharmacology, Toxicology and Pharmaceutics (Life Sciences)