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Cloning, tissue expression, and inducibility of CYP 3A79 from sea bass (Dicentrarchus labrax) by E. Vaccaro; A. Salvetti; R. Del Carratore; S. Nencioni; V. Longo; P. G. Gervasi is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Cloning, tissue expression, and inducibility of CYP 3A79 from sea bass (Dicentrarchus labrax) about?

## Abstract Multiple members of the CYP3A subfamily have been identified and intensively studied in mammals as they represent prominent CYP enzymes involved in drug metabolism. Also in fish, some CYP3A genes have been identified by cDNA cloning and immunological techniques, but relatively little is known about their function, distribution, and inducibility. In this study, a novel CYP3A, designated as CYP3A79 was isolated from adult male sea bass, an economically valuable species in fisheries. The sea bass CYP3A79 that was cloned contained an open‐reading frame of 1512 bp that encoded a 504 amino acid protein and shared a high‐sequence identity with medaka, killifish, and trout CYP3As. Interestingly, CYP3A79 also shares five of six substrate recognition sites (SRS) with the SRS of other fish CYP3As, suggesting an evolutionary conservation of the function of these enzymes. In this fish, we also investigated the expression of CYP3A79 and its susceptibility to induction by various compounds including clotrimazole and dehydroepiandrosterone, two strong ligands of zebrafish PXR. The expression of CYP3A79 mRNA was detected by RT‐PCR only in the intestine and liver. The immunoblot analysis

Who reads Cloning, tissue expression, and inducibility of CYP 3A79 from sea bass (Dicentrarchus labrax)?

Author

E. Vaccaro; A. Salvetti; R. Del Carratore; S. Nencioni; V. Longo; P. G. Gervasi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1095-6670)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)