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Plant Genomic DNA Extraction Protocol by Prayash Nayak is a document available to read on EtoBox.

What is Plant Genomic DNA Extraction Protocol about?

This document outlines a procedure to isolate genomic DNA from plant tissues using the CTAB method. Key steps include: 1) Disrupting plant cell walls and membranes using an extraction buffer containing CTAB detergent, EDTA, and NaCl to release DNA. 2) Separating DNA from other cell components like proteins and polysaccharides by chloroform extraction and precipitation using potassium acetate and isopropanol. 3) Isolating the precipitated DNA pellet and dissolving it in TE buffer for storage. The overall

Author

Prayash Nayak

Language

EN