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The role of β93 Cys in the inhibition of Hb S fiber formation by Kelly M. Knee; Catherine K. Roden; Mark R. Flory; Ishita Mukerji is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The role of β93 Cys in the inhibition of Hb S fiber formation about?
Recent studies have suggested that nitric oxide (NO) binding to hemoglobin (Hb) may lead to the inhibition of sickle cell fiber formation and the dissolution of sickle cell fibers. NO can react with Hb in at least 3 ways: 1) formation of Hb(II)NO, 2) formation of methemoglobin, and 3) formation of S-nitrosohemoglobin, through nitrosylation of the β93 Cys residue. In this study, the role of β93 Cys in the mechanism of sickle cell fiber inhibition is investigated through chemical modification with N-ethylmaleimide. UV resonance Raman, FT-IR and electrospray ionization mass spectroscopic methods in conjunction with equilibrium solubility and kinetic studies are used to characterize the effect of β93 Cys modification on Hb S fiber formation. Both FT-IR spectroscopy and electrospray mass spectrometry results demonstrate that modification can occur at both the β93 and α104 Cys residues under relatively mild reaction conditions. Equilibrium solubility measurements reveal that singlymodified Hb at the β93 position leads to increased amounts of fiber formation relative to unmodified or doubly-modified Hb S. Kinetic studies confirm that modification of only the β93 residue leads to a faster
Who reads The role of β93 Cys in the inhibition of Hb S fiber formation?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kelly M. Knee; Catherine K. Roden; Mark R. Flory; Ishita Mukerji
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0301-4622)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)