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Simultaneous Determination of Tocopherols, Ubiquinols, and Ubiquinones in Blood, Plasma, Tissue Homogenates, and Subcellular Fractions by Johanna K. Lang; Kishorchandra Gohil; Lester Packer is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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A fast single-step lipid extraction procedure and high-performance liquid chromatography with in-line uv and electrochemical detection are used for the simultaneous quantitative determination of tocopherols, ubiquinols, and ubiquinones in blood, plasma, tissue homogenates, and subcellular fractions. The compounds of interest can be quantitatively extracted into hexane from a sodium dodecyl sulfate-treated aqueous homogenate after precipitation of protein by addition of an equal volume of ethanol. (Y-, y-, and &Tocopherol, ubiquino19, ubiquinol 10, and ubiquinones 9 and 10 can be well separated on a reversed phase column. Ubiquinones are detected at 275 nm by the uv detector, and ubiquinols and tocopherols by the electrochemical detector in the oxidative mode. Quantitation is done by comparing chromatographic peak heights to those. of a standard solution containing known amounts of tocopherols, ubiquinols 9 and 10, and ubiquinones 9 and 10, analyzed under identical conditions. The high sensitivity of the electrochemical detection allows operation at low potentials (+0.5 V) with low detector response, but high selectivity for the easily oxidizable tocopherols and ubiquinols and decre

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Author

Johanna K. Lang; Kishorchandra Gohil; Lester Packer

Publisher

Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)

Published

1986

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)