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Gastric neuroendocrine neoplasms: tumour clonality and malignancy-associated large X-chromosomal deletions by D'Adda, Tiziana; Candidus, Sonja; Denk, Helmut; Bordi, Cesare; Hfler, Heinz is a Medicine article available to read on EtoBox.

What is Gastric neuroendocrine neoplasms: tumour clonality and malignancy-associated large X-chromosomal deletions about?

Type I gastric carcinoid tumours associated with corporal (body of stomach) atrophic gastritis (CAG) are benign tumours developing as the final step of a hyperplastic precursor sequence. The neoplastic nature of these tumours has been assumed but never proved. Type III gastric carcinoid tumours and neuroendocrine carcinomas are malignant neoplasms without known precursor lesions. To assess the neoplastic nature of type I carcinoids, the clonal status of 35 tumours from 23 female patients was investigated using the human androgen receptor (HUMARA) gene test, which is based on the pattern of X-chromosome inactivation. For comparison, the same test was also performed on four type III carcinoids and two neuroendocrine carcinomas. DNA extracted from paraffin sections was digested with HhaI restriction enzyme and then amplified by polymerase chain reaction (PCR) using established HUMARA primers. The PCR products were analysed in an automated DNA sequencer. In a complementary analysis of the same tumours, loss of heterozygosity (LOH) on the X chromosome was studied using three polymorphic markers (DXS989, DXS1003, DXS1192) in a PCR-microsatellitebased technique. After exclusion of non-inf

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Author

D'Adda, Tiziana; Candidus, Sonja; Denk, Helmut; Bordi, Cesare; Hfler, Heinz

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3417)

Published

1999

Language

EN

Field

Medicine (Health Sciences)