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Comparison of Different Protocols for Neural Differentiation of Human Induced Pluripotent Stem Cells by Ali Salimi; Samad Nadri; Marzieh Ghollasi; Khosro Khajeh; Masoud Soleimani is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Comparison of Different Protocols for Neural Differentiation of Human Induced Pluripotent Stem Cells about?

Although embryonic stem cells (ESCs) have enormous potentials due to their pluripotency, their therapeutic use is limited by ethical, biological and safety issues. Compared to ESCs, induced pluripotent stem cells (iPSCs) can be obtained from mouse or human fibroblasts by reprogramming. Numerous studies have established many protocols for differentiation of human iPSCs (hiPSCs) into neural lineages. However, the low differentiation efficiency of such protocols motivates researchers to design new protocols for high yield differentiation. Herein, we compared neural differentiation potential of three induction media for conversion of hiPSCs into neural lineages. In this study, hiPSCs-derived embryoid bodies were plated on laminin coated dishes and were treated with three induction media including (1) bFGF, EGF (2) RA and (3) forskolin, IBMX. Immunofluorescence staining and quantitative real-time PCR (qPCR) analysis were used to detect the expression of neural genes and proteins. qPCR analysis showed that the expression of neural genes in differentiated hiPSCs in forskolin, IBMX supplemented media was significantly higher than undifferentiated cells and those in induction media containi

Who reads Comparison of Different Protocols for Neural Differentiation of Human Induced Pluripotent Stem Cells?

Author

Ali Salimi; Samad Nadri; Marzieh Ghollasi; Khosro Khajeh; Masoud Soleimani

Publisher

Springer Science and Business Media LLC

Published

2014

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)