Can I read Influence of Hydroxyl Groups on the Biological Properties of Cationic Polymethacrylates as Gene Vectors on EtoBox?

Influence of Hydroxyl Groups on the Biological Properties of Cationic Polymethacrylates as Gene Vectors by Ming Ma; Feng Li; Zhe-fan Yuan; Ren-xi Zhuo is a scholarly article available to read on EtoBox.

What is Influence of Hydroxyl Groups on the Biological Properties of Cationic Polymethacrylates as Gene Vectors about?

In this study poly(aminoethyl methacrylate) (PAEMA), poly(3-amino-2-hydroxypropyl methacrylate) (PAHPMA), poly(2-(2-aminoethylamino)ethyl methacrylate) (PAEAEMA) and poly(3-(2-aminoethylamino) 2-hydroxypropyl methacrylate) (PAEAHPMA) were synthesized using atom transfer radical polymerization to evaluate the effect of hydroxyl groups on the relative properties of cationic polymeric gene vectors. The results of heparin displacement assays showed that PAHPMA possessed a stronger binding capacity than PAEMA. PAHPMA/DNA complexes and PAEAHPMA/DNA complexes had lower zeta potentials than those of PAEMA and PAEAEMA. MTT assay results indicated that PAHPMA and PAEAHPMA exhibited obviously lower cytotoxicities than PAEMA and PAEAEMA. Subsequently, in vitro gene transfection studies in 293T cells without serum showed that PAHPMA exhibited a lower transfection efficiency than PAEMA and PAEAHPMA/DNA complexes possessed a similar transfection efficiency to PAEAEMA/DNA complexes. Moreover, PAHPMA and PAEAHPMA retained similar transfection efficiencies in DMEM with 10% serum, but PAEMA and PAEAEMA showed slightly lower transfection efficiencies than in the absence of serum. The reason for these

Author

Ming Ma; Feng Li; Zhe-fan Yuan; Ren-xi Zhuo

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1742-7061)

Published

2010

Language

EN