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Protocol for glial Ca^2+^ imaging in C. elegans following chemical, mechanical, or optogenetic stimulation by Hankui Cheng; Umar Al-Sheikh; Du Chen; Duo Duan; Lijun Kang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Protocol for glial Ca^2+^ imaging in C. elegans following chemical, mechanical, or optogenetic stimulation about?
## Summary __Caenorhabditis elegans__ is an exceptionally transparent model to analyze calcium (Ca^2+^) signals, but available protocols for neuronal Ca2^+^ imaging may not be suitable for studying glial cells. Here, we present a detailed protocol for glial Ca^2+^ imaging in __C. elegans__ following three different approaches including chemical, mechanical, and optogenetic stimulation. We also provide the details for imaging analysis using Image-J. For complete details on the use and execution of this protocol, please refer to Duan et al. (2020).
Who reads Protocol for glial Ca^2+^ imaging in C. elegans following chemical, mechanical, or optogenetic stimulation?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hankui Cheng; Umar Al-Sheikh; Du Chen; Duo Duan; Lijun Kang
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)