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The Effect of Endosomal Escape Peptides on in Vitro Gene Delivery of Polyethylene Glycol‐based Vehicles by Nicole M. Moore; Clayton L. Sheppard; Tiffany R. Barbour; Shelly E. Sakiyama‐Elbert is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The Effect of Endosomal Escape Peptides on in Vitro Gene Delivery of Polyethylene Glycol‐based Vehicles about?
## Abstract ## Background With recent progress in gene therapy clinical trials, there is an even greater demand to advance the development of nonviral gene delivery vehicles. We have previously developed poly(ethylene glycol) (PEG)‐based vehicles with transfection efficiency similar to polyethyleneimine and low cytotoxicity. It was hypothesized that conjugating endosomal escape peptides (EEPs) to PEG‐based vehicles would further increase their transfection efficiency. The present study aimed to determine how two different EEPs, INF7 and H5WYG, which destabilize the endosomal membrane at different pHs, affect the efficiency of PEG‐based vehicles. ## Methods INF7 and H5WYG were conjugated to PEG‐tetraacrylate (PEG‐TA) via a Michael‐type addition at the desired molar ratios. The pH‐dependent membrane lytic activity, transfection efficiency, particle size, zeta potential, and endosomal escape kinetic rate constants were determined. ## Results Fusogenic peptides, INF7 and H5WYG, showed pH‐dependent membrane lytic activity when conjugated to PEG‐TA. The highest membrane lytic activity of PEG‐INF7 and PEG‐H5WYG conjugates occurred at pH 5 and 5.5, respectively. Coupling one INF7 peptide t
Who reads The Effect of Endosomal Escape Peptides on in Vitro Gene Delivery of Polyethylene Glycol‐based Vehicles?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Nicole M. Moore; Clayton L. Sheppard; Tiffany R. Barbour; Shelly E. Sakiyama‐Elbert
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)
- Published
- 2008
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)