Opening book details…
Can I read N ‐Acetyl α‐D‐Glucosaminidase from the Venom of African Puff Adder ( Bitis Arietans ) on EtoBox?
N ‐Acetyl α‐D‐Glucosaminidase from the Venom of African Puff Adder ( Bitis Arietans ) by Andrew J. Nok; M. N. Shuaibu; M. K. Choudhry; O. Oyebanjo; S. Ibrahim; S. Williams is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is N ‐Acetyl α‐D‐Glucosaminidase from the Venom of African Puff Adder ( Bitis Arietans ) about?
## Abstract The activity of __N__‐acetyl‐α‐D‐glucosaminidase from venom of the African puff adder (__Bitis arietans__) has been detected. The enzyme from the venom was purified by chromatography on Q‐sepharose, CM‐cellulose, and __N__‐acetyl‐α‐D‐glucosamine‐agarose affinity column. The enzyme has a molecular weight of 102 kDa determined by size exclusion chromatography on Sephacryl 200. It migrated as a 51‐kDa band on SDS polyacrylamide gels. The enzyme is maximally active at pH 5.5 and 40^°^C. The __B. arietans__ NAGase hydrolyzed exclusively terminally linked α‐(1–4) GlcNAc residues from nonreducing ends of oligosaccharides. It hydrolysed chito‐oligosaccharide, MU‐GlcNAc and chitobiose with __K__~M~ values of 0.15 mM and 1.22 mM, respectively. Swollen chitin and oligosaccharide above (GlcNAc)~4~ were not hydrolysed by the enzyme. __B. arietans__ NAGase was strongly inhibited noncompetitively by Hg^2+^, competitively by 1‐thio‐β‐D‐GlcNAc and __N__‐acetyl glucosamine (NAG) with __K__~i~ of 0.55, 0.25 and 8 mM, respectively. Colombin the active component of antivenom preparation from __Aristolodia albida__ inhibited the enzyme competitively with __K__~i~ of 0.6 mM. Delineation of th
Who reads N ‐Acetyl α‐D‐Glucosaminidase from the Venom of African Puff Adder ( Bitis Arietans )?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Andrew J. Nok; M. N. Shuaibu; M. K. Choudhry; O. Oyebanjo; S. Ibrahim; S. Williams
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1095-6670)
- Published
- 2001
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)