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Cloning Human Fibroblasts from Biopsies by FELIPE HELFENSTEIN is a document available to read on EtoBox.

This chapter outlines methods for establishing, maintaining, and cloning human primary cell strains, particularly fibroblasts from skin biopsies of normal boys and Duchenne muscular dystrophy carriers. It discusses the importance of collaboration with clinical colleagues for biopsy collection and the use of specific materials and techniques for cell culture and cloning. The chapter also details protocols for primary explant culture, subculture, and cell zoning to ensure successful cell growth and cloning.

Author

FELIPE HELFENSTEIN

Language

EN