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Connexin43 in Rat Pituitary: Localization at Pituicyte and Stellate Cell Gap Junctions and Within Gonadotrophs by T. Yamamoto; M. Z. Hossain; E. L. Hertzberg; H. Uemura; L. J. Murphy; J. I. Nagy is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Immunohistochemical methods were employed to investigate the cellular and ultrastructural localization of the gap junction protein connexin43 (Cx43) in rat pituitary. Western blots of pituitary homogenates probed with anti-Cx43 antibodies showed the presence of Cx43 in both anterior and posterior pituitary lobes. By light microscopy (LM), Cx43-immunoreactive (Cx43-IR) puncta were found in all areas of the posterior lobe, but at greater concentrations in peripheral regions of this structure. By electron microscopy (EM), immunogold labelling for Cx43 was seen at gap junctions between thin cytoplasmic processes of pituicytes. No immunoreactivity was detected in the intermediate lobe. The anterior lobe contained puncta similar to but more sparsely scattered than those in the posterior lobe, and by EM analysis these were demonstrated to correspond to labelled gap junctions between stellate cells. In addition, anti-Cx43 antibodies produced intracellular labelling in a small percentage of endocrine cells, which were distributed throughout the anterior lobe and determined by double immunostaining methods to be cells containing luteinizing hormone. By EM, labelling within these cells was as

Who reads Connexin43 in Rat Pituitary: Localization at Pituicyte and Stellate Cell Gap Junctions and Within Gonadotrophs?

Author

T. Yamamoto; M. Z. Hossain; E. L. Hertzberg; H. Uemura; L. J. Murphy; J. I. Nagy

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 1432-119X)

Published

1993

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)