About this document
Protein Purification Techniques Guide by Melwin Mejan is a document available to read on EtoBox.
This document provides information on the process of purifying proteins. It discusses that purified proteins are needed to determine amino acid sequences and investigate biochemical functions. The main steps described for protein purification are homogenization, centrifugation, precipitation, dialysis, chromatography, electrophoresis, and mass spectroscopy. Chromatography techniques discussed include gel filtration, ion exchange, affinity, and HPLC which separate proteins based on size, charge, and binding
- Author
- Melwin Mejan
- Language
- EN