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Enhanced retention of in vitro functional activity of platelets from recombinant human thrombopoietin‐treated patients following long‐term cryopreservation with a platelet‐preserving solution (ThromboSol) and 2% DMSO by Saroj Vadhan‐Raj; Laura M. Currie; Carlos Bueso‐Ramos; Stephen A. Livesey; Jerome Connor is a Medicine article available to read on EtoBox.

What is Enhanced retention of in vitro functional activity of platelets from recombinant human thrombopoietin‐treated patients following long‐term cryopreservation with a platelet‐preserving solution (ThromboSol) and 2% DMSO about?

Chemotherapy‐induced thrombocytopenia represents a significant clinical problem in the management of patients with malignancy. Recombinant human thrombopoietin (rhTPO) is a potent stimulator of platelet production __in vivo__. The ability to cryopreserve rhTPO‐derived platelets would enable the use of autologous platelets during the period of thrombocytopenia. ThromboSol^TM^ is a platelet‐stabilizing formulation consisting of second messenger effectors that inhibit specific activation pathways endogenous to platelets. To investigate the effect of ThromboSol cryopreservation, platelets from rhTPO‐treated patients (__n__ = 23) and normal donors were treated with ThromboSol and 2% DMSO and cryopreserved for up to 6 months. The platelets were thawed at different intervals and tested for retention of platelet functional activity __in vitro__. Following a short‐term storage (1 week), the cryopreserved platelets from patients treated with rhTPO exhibited significantly higher retention of functional activities including discoid morphology (70% __v__ 57%), extent of shape change (19% __v__ 13%) stirring shape change (15% __v__ 11%) and hypotonic shock response (56% __v__ 25%), as compared t

Who reads Enhanced retention of in vitro functional activity of platelets from recombinant human thrombopoietin‐treated patients following long‐term cryopreservation with a platelet‐preserving solution (ThromboSol) and 2% DMSO?

Author

Saroj Vadhan‐Raj; Laura M. Currie; Carlos Bueso‐Ramos; Stephen A. Livesey; Jerome Connor

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0007-1048)

Published

1999

Language

EN

Field

Medicine (Health Sciences)