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Determination of Eight Sulfonamides in Bovine Kidney by Liquid Chromatography/tandem Mass Spectrometry with On-line Extraction and Sample Clean-up by Nico Van Eeckhout; José Castro Perez; Carlos Van Peteghem is a Chemistry article available to read on EtoBox.

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A sensitive, high performance liquid chromatography/tandem mass spectrometric (i.e. mass spectrometry/ mass spectrometry; LC/MS/MS) method with on-line extraction and sample clean-up for the screening and confirmation of residues of sulfonamides in kidney is described. The sulfonamides are extracted from homogenized kidney with methanol. After centrifugation of the extract, an aliquot of the extract is directly injected on the LC/MS/MS system with further extraction and clean-up of the sample on-line. Detection of the analytes was achieved by positive electrospray ionization (ESI) followed by multiple reaction monitoring. For each sulfonamide the collisional decomposition of the protonated molecule to a common, abundant fragment ion was monitored. The method has been validated for sulfadimethoxine, sulfaquinoxaline, sulfamethazine, sulfamerazine, sulfathiazole, sulfamethoxazole, sulfadiazine and sulfapyridine. Calibration curves resulting from spiked blank kidney samples at the 10-200 mg/kg level showed good linear correlation. At the level of 50, 100 and 200 mg/kg both within-and between-day precision, as measured by relative standard deviation (RSD), were less than 16%. The limit

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Author

Nico Van Eeckhout; José Castro Perez; Carlos Van Peteghem

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2000

Language

EN

Field

Chemistry (Physical Sciences)