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Influence of zinc deficiency on the mRNA expression of zinc transporters in adult rats by Michael W. Pfaffl; Wilhelm Windisch is a Environmental Science article available to read on EtoBox.

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The accumulation of zinc in the cell is a sum of influx and efflux processes via transporter proteins, like the four Zn transporters (ZnT1-4), the divalent cation transporter 1 (DCT1) and of storage processes mainly bound to metallothionein (MT). To study the effect of Zn deficiency on mRNA expression levels, adult rats were used as an animal mode[. Food intake was restricted to 8 g/day containing 2 IJg Zn/g fortified with pure phytate in Zn deficiency rats and 58 IJg Zn/g in controls (n = 7). At day 1, 2, 4, 7, 11, 16, 22, and 29 of Zn deficiency, 3 animals were sacrificed, respectively (n = 24). Zn deficiency was evident from reduced plasma Zn, plasma alkaline phosphatase activity and severe mobilization of Zn from tissue stores (mainly skeleton), while food intake and body weight remained unaffected. Tissues representing Zn absorption (jejunum, coLon), Zn storage and utilization (muscle, Liver), and Zn excretion (kidney) were retrieved. Total RNA contents increased in colon (p = 0.003) and trend to decrease in Uver (p = 0.086). Zn deficiency was without effect on tissue total RNA concentrations in muscle tissue and kidney. ReaL-time reverse transcription (RT) polymerase chain re

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Author

Michael W. Pfaffl; Wilhelm Windisch

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0946-672X)

Published

2003

Language

EN

Field

Environmental Science (Physical Sciences)