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Nuclear accumulation and activation of p53 in embryonic stem cells after DNA damage by Valeriya Solozobova; Alexandra Rolletschek; Christine Blattner is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Nuclear accumulation and activation of p53 in embryonic stem cells after DNA damage about?
## Abstract ## Background P53 is a key tumor suppressor protein. In response to DNA damage, p53 accumulates to high levels in differentiated cells and activates target genes that initiate cell cycle arrest and apoptosis. Since stem cells provide the proliferative cell pool within organisms, an efficient DNA damage response is crucial. ## Results In proliferating embryonic stem cells, p53 is localized predominantly in the cytoplasm. DNA damage-induced nuclear accumulation of p53 in embryonic stem cells activates transcription of the target genes __mdm2__, __p21__, __puma__ and __noxa__. We observed bi-phasic kinetics for nuclear accumulation of p53 after ionizing radiation. During the first wave of nuclear accumulation, p53 levels were increased and the p53 target genes __mdm2__, __p21__ and __puma__ were transcribed. Transcription of __noxa__ correlated with the second wave of nuclear accumulation. Transcriptional activation of p53 target genes resulted in an increased amount of proteins with the exception of p21. While p21 transcripts were efficiently translated in 3T3 cells, we failed to see an increase in p21 protein levels after IR in embryonal stem cells. ## Conclusion In embr
Who reads Nuclear accumulation and activation of p53 in embryonic stem cells after DNA damage?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Valeriya Solozobova; Alexandra Rolletschek; Christine Blattner
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-2018.; Springer Science and Business Media LLC (ISSN 1471-2121)
- Published
- 2009
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)