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Comparison of DNA methylation levels of repetitive loci during bovine development by Masahiro Kaneda; Satoshi Akagi; Shinya Watanabe; Takashi Nagai is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract ## Background DNA methylation of cytosine residues in CpG dinucleotide controls gene expression and dramatically changes during development. Its pattern is disrupted in cloned animals suggesting incomplete reprogramming during somatic cell nuclear transfer (the first reprogramming). However, the second reprogramming occurs in the germ cells and epigenetic errors in somatic cells of cloned animals should be erased. To analyze the DNA methylation changes on the spermatogenesis of bulls, we measured DNA methylation levels of three repetitive elements in blastocysts, blood and sperm. ## Methods DNA from PBLs (peripheral blood leukocytes), sperm and individual IVF (__in vitro__ fertilized) and parthenogenetic blastocysts was isolated and bisulfite converted. Three repetitive elements; Satellite I, Satellite II and __art2__ sequences were amplified by PCR with specific pairs of primers. The PCR product was then cut by restriction enzymes and analyzed by agarose gel electrophoresis for determining the DNA methylation levels. ## Results Both Satellite I and Satellite II sequences were highly methylated in PBLs, whereas hypo-methylated in sperm and blastocysts. The __art2__ seq

Who reads Comparison of DNA methylation levels of repetitive loci during bovine development?

Author

Masahiro Kaneda; Satoshi Akagi; Shinya Watanabe; Takashi Nagai

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2007-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1753-6561)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)