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This document describes the development of a bioanalytical LC-MS/MS method for the determination of ethinyl estradiol in human plasma. Ethinyl estradiol was derivatized with dansyl chloride to improve ionization and detection by mass spectrometry. Calibration standards and quality control samples were prepared over a range of 2-500 pg/mL. The chromatographic method was optimized on a C18 column using an isocratic mobile phase of methanol and 5mM ammonium formate at a flow rate of 0.3 mL/min. This method pro

Author

siddiqui.fahad884

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EN