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A survey of the response of Rhododendron to in vitro culture by Brent H. McCown; Gregory B. Lloyd is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The responses of 7 genotypes of Rhodendron to culture conditions and their establishment as shoot cultures are described. The genotypes represent a broad genetic diversity in the genus. After sterilization and an acclimation period of 3 to 12 months, all tile selections were established as shoot cultures on Woody Plant Medium (WPM) supplemented with N 6 -(A 2 -isopentenyl) adenine (2iP). Plants with strong episodic growth cycles required the longer acclimation periods. Utilizing shoots from these cultures, the response to a cytokinin series of 0 to 32tzM 2iP or BAP (6-Benzylaminopurine) was analyzed. BAP proved toxic to all but the elipidote and lepidote rhododendrons (R. mucronulatum, R. × 'Boule de Neige', and R. X 'PJM'); however, even with these selections, 2iP stimulated greater shoot multiplication rates. The optimum 2iP level for shoot multiplication varied little with the genotype and levels of 4 to 16 tzM generally proved optimal, depending on the specific selection. Adventitious shoot production was observed in 3 selections (R. canadense, R. × 'Boule de Neige' and R. × 'PJM'), but only at 2iP levels above 8t~M. Shoot multiplication rates of 7 to 21 times were observed, de

Who reads A survey of the response of Rhododendron to in vitro culture?

Author

Brent H. McCown; Gregory B. Lloyd

Publisher

Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0167-6857)

Published

1982

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)