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Regulatory action of prolactin on the in vitro growth of CD34+ve human hemopoietic progenitor cells by Graziella Bellone; Massimo Geuna; Anna Carbone; Stefania Silvestri; Robin Foa; Giorgio Emanuelli; Lina Matera is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The pituitary hormone prolactin (PrI) is known to act as a local regulator of immune cell function, and Prl-binding receptors (Prl-R) have been described to share distinctive features with the members of the newly described cytokine/ hemopoietin receptor superfamily. Here we show that the hormone can functionally interact with lineage-specific hemopoietic factors. When highly purified progenitor cells (CD34+ve) were seeded in semisolid methylcellulose cultures in the presence of interleukin (IL)-3, granulocyte-macrophage colony stimulating factor (GM-CSF), and erythropoietin (Epo), a selective enhancing effect of Prl on the formation of colony forming unit-granulocyte (CFU-G) and burst forming uniterythroid (BFU-E) colonies was observed. The effect of the hormone was plotted as a bell shaped curve, with the optimal response at the supraphysiological concentration of 50 ng/ml. Limiting dilution analysis showed that Prl acted directly on hemopoietic progenitors. This was confirmed by the observation on the CD34+ve cells of Prl-binding sites reacting with the specific monoclonal antibodies (mAbs), U5 and PrR-7A. lmmunoprecipitation of the metabolically labeled CD34+ve cells with the P

Who reads Regulatory action of prolactin on the in vitro growth of CD34+ve human hemopoietic progenitor cells?

Author

Graziella Bellone; Massimo Geuna; Anna Carbone; Stefania Silvestri; Robin Foa; Giorgio Emanuelli; Lina Matera

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1995

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)