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Chemical and thermal denaturation of crystalline bacterial S-layer proteins: An atomic force microscopy study by José L. Toca-Herrera; Susana Moreno-Flores; Jacqueline Friedmann; Dietmar Pum; Uwe B. Sleytr is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Crystalline monomolecular cell surface layers, S‐layers, are one of the most common outermost cell envelope components of the prokaryotic organisms (bacteria and archaeda) that protects them from competitive habitats. Since isolated S‐protein subunits are able to re‐assemble into crystalline arrays on lipid films and solid supports making biomimetic surfaces, S‐layer technology is currently used in nanobiotechnology. An important aspect of the biomimetic surfaces built with S‐layers is their stability under extreme solvent conditions or temperature. Chemical (pH, alcohol) and physical (thermal) denaturant conditions were employed to test the stability of S‐layers. Recrystallized bacterial surface layers from __Bacillus sphaericus__ (SbpA) on hydrophilic silicon wafers loses the crystalline structure at 80% ethanol/water mixtures, the change in structure being reversible after treating the surface with buffer solution. SbpA on silicon supports denatures at pH 3 and at 70°C, and the process is irreversible. Cross‐linking of SbpA enhances the stability for high ethanol and acidic conditions, but it does not improve thermal stability. Recrystallized SbpA on secondary cell w

Who reads Chemical and thermal denaturation of crystalline bacterial S-layer proteins: An atomic force microscopy study?

Author

José L. Toca-Herrera; Susana Moreno-Flores; Jacqueline Friedmann; Dietmar Pum; Uwe B. Sleytr

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1059-910X)

Published

2004

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)