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Sustained Wnt protein expression in chondral constructs from mesenchymal stem cells by Katsuhiro Nishioka; James E. Dennis; Jizong Gao; Victor M. Goldberg; Arnold I. Caplan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Sustained Wnt protein expression in chondral constructs from mesenchymal stem cells about?
## Abstract Wnt genes encode a number of secreted glycoproteins which are closely associated with the cell surface and the extracellular matrix. Recently, members of Wnt family have been implicated in regulating chondrocyte differentiation, but their roles in the chondrogenic process are not fully understood. To contribute to an understanding of the roles of Wnts during chondrogenesis, we have analysed the spatiotemporal expression patterns of Wnt using in vitro models for chondrogenesis of human bone marrow‐derived mesenchymal stem cells (hMSCs). In chondrogenic aggregate culture system, RT‐PCR analysis revealed expression of Wnt5a and Wnt4 during late chondrogenesis (days 10 and 15). Immunohistochemical analysis showed widespread distribution of Wnt5a and Wnt4 throughout the aggregates at this late phase of culture (days 14 and 21). In addition, in this aggregate culture system, immunohistochemical staining of Wnt4 and Wnt5a showed similar spatiotemporal expression patterns to that of type II collagen or type X collagen. To confirm the results obtained by immunostaining, the specificity of the anti‐Wnt4 or anti‐Wnt5a antibody was assessed by Western blot analysis. Of Wnt4 and Wnt
Who reads Sustained Wnt protein expression in chondral constructs from mesenchymal stem cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Katsuhiro Nishioka; James E. Dennis; Jizong Gao; Victor M. Goldberg; Arnold I. Caplan
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2004
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)