About this Biochemistry, Genetics and Molecular Biology article

Optimization of Enzyme Complexes for Lignocellulose Hydrolysis by Alex Berlin; Vera Maximenko; Neil Gilkes; Jack Saddler is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

## Abstract The ability of a commercial __Trichoderma reesei__ cellulase preparation (Celluclast 1.5L), to hydrolyze the cellulose and xylan components of pretreated corn stover (PCS) was significantly improved by supplementation with three types of crude commercial enzyme preparations nominally enriched in xylanase, pectinase, and β‐glucosidase activity. Although the well‐documented relief of product inhibition by β‐glucosidase contributed to the observed improvement in cellulase performance, significant benefits could also be attributed to enzymes components that hydrolyze non‐cellulosic polysaccharides. It is suggested that so‐called “accessory” enzymes such as xylanase and pectinase stimulate cellulose hydrolysis by removing non‐cellulosic polysaccharides that coat cellulose fibers. A high‐throughput microassay, in combination with response surface methodology, enabled production of an optimally supplemented enzyme mixture. This mixture allowed for a ∼twofold reduction in the total protein required to reach glucan to glucose and xylan to xylose hydrolysis targets (99% and 88% conversion, respectively), thereby validating this approach towards enzyme improvement and process cost

Author

Alex Berlin; Vera Maximenko; Neil Gilkes; Jack Saddler

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2006

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)