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Comparative Immunochemical Study of Ribosomal Proteins from Various Mammalian Cells by Two-dimensional Immunoelectrophoresis by Marie-Annick Auger-Buendia; Marie-Paule Ollier-Hartmann; Armand Tavitian; Lucien Hartmann is a scholarly article available to read on EtoBox.

What is Comparative Immunochemical Study of Ribosomal Proteins from Various Mammalian Cells by Two-dimensional Immunoelectrophoresis about?

Proteins isolated from ribosomal subunits of various mammalian cells were analysed comparatively by two different methods: a two-dimensional polyacrylamide gel electrophoresis system and a recently described two-dimensional immunoelectrophoresis technique. For this purpose, antisera were raised in rabbits against the total mixture of ribosomal proteins from murine cells. These sera were characterized by ring-test, double immunodiffusion and two-dimensional immunoelectrophoresis. They were shown to contain antibodies to a large number of ribosomal proteins. Immunoelectrophoretic analysis of 60S and 40S subunit proteins from rabbit, lamb, canine and human cells using anti-murine sera revealed a striking conservation of their antigenic properties. These results corroborated those obtained by two-dimensional polyacrylamide gel electrophoresis.

Author

Marie-Annick Auger-Buendia; Marie-Paule Ollier-Hartmann; Armand Tavitian; Lucien Hartmann

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 0026-8925)

Published

1981

Language

EN