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Can I read Roles of Extracellular Matrix Components in Differentiating Teratocarcinoma Cells on EtoBox?
Roles of Extracellular Matrix Components in Differentiating Teratocarcinoma Cells by A Grover; E D Adamson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Roles of Extracellular Matrix Components in Differentiating Teratocarcinoma Cells about?
F9 embryonal carcinoma cells treated with 5 X 10(-8) M retinoic acid and cultured in suspension for 8 days form aggregates consisting of an outer epithelial layer of alpha-fetoprotein-producing visceral endoderm cells. We have previously shown (Grover, A., Oshima, R. G., and Adamson, E. D. (1983) J. Cell Biol. 96, 1690-1696) that the differentiation of F9 cells to visceral endoderm is accompanied by the activation of several genes, and increased laminin synthesis is one of the earliest events. Here we analyze in detail the syntheses and secretion of fibronectin, type IV collagen, and laminin during the 8-day process. Employing immunoprecipitation and enzyme-linked immunosorbent assay, we show that the levels of all three components change with different patterns. Unstimulated F9 cells synthesize and secrete relatively high levels of fibronectin and low levels of type IV collagen. Fibronectin synthesis and secretion decreases to 10% of its original level whereas type IV collagen synthesis rises approximately 3-fold during the differentiation process. Laminin synthesis also rises at least 2-fold, and the proportions of its subunits change as the syntheses of B1 and A accelerate start
Who reads Roles of Extracellular Matrix Components in Differentiating Teratocarcinoma Cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- A Grover; E D Adamson
- Publisher
- Elsevier BV
- Published
- 1985
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)