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Validation of an Assay for the Determination of Cotinine and 3-Hydroxycotinine in Human Saliva Using Automated Solid-phase Extraction and Liquid Chromatography with Tandem Mass Spectrometric Detection by Mark C. Bentley; Mohammed Abrar; Mark Kelk; Jeremy Cook; Keith Phillips is a Chemistry article available to read on EtoBox.
What is Validation of an Assay for the Determination of Cotinine and 3-Hydroxycotinine in Human Saliva Using Automated Solid-phase Extraction and Liquid Chromatography with Tandem Mass Spectrometric Detection about?
The validation of a high-performance liquid chromatographic method for the simultaneous determination of low level cotinine and 3-hydroxycotinine in human saliva is reported. Analytes and deuterated internal standards were extracted from saliva samples using automated solid-phase extraction, the columns containing a hyper cross-linked styrene-divinylbenzene copolymer sorbent, and analysed by reversed-phase liquid chromatography with tandem mass spectrometric detection (LC-MS-MS). Lower limits of quantitation of 0.05 and 0.10 ng / ml for cotinine and 3-hydroxycotinine, respectively, were achieved. Intra-and inter-batch precision and accuracy values fell within 617% for all quality control samples, with the exception of quality control samples prepared at 0.30 ng / ml for 3-hydroxycotinine (inter-day precision 21.1%). Results from the analysis of saliva samples using this assay were consistent with subjects' self-reported environmental tobacco smoke (ETS) exposures, enhancing the applicability of cotinine as a biomarker for the assessment of low level ETS exposure.
Who reads Validation of an Assay for the Determination of Cotinine and 3-Hydroxycotinine in Human Saliva Using Automated Solid-phase Extraction and Liquid Chromatography with Tandem Mass Spectrometric Detection?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Mark C. Bentley; Mohammed Abrar; Mark Kelk; Jeremy Cook; Keith Phillips
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0378-4347)
- Published
- 1999
- Language
- EN
- Field
- Chemistry (Physical Sciences)