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A functional interaction between GRP78 and Zika virus E protein by Khongwichit, Sarawut (author);Sornjai, Wannapa (author);Jitobaom, Kunlakanya (author);Greenwood, Mingkwan (author);Greenwood, Michael P. (author);Hitakarun, Atitaya (author);Wikan, Nitwara (author);Murphy, David (author);Smith, Duncan R. (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is A functional interaction between GRP78 and Zika virus E protein about?

## Abstract Zika virus (ZIKV) is a mosquito-transmitted virus that has caused significant public health concerns around the world, partly because of an association with microcephaly in babies born to mothers who were infected with ZIKV during pregnancy. As a recently emerging virus, little is known as to how the virus interacts with the host cell machinery. A yeast-2-hybrid screen for proteins capable of interacting with the ZIKV E protein domain III, the domain responsible for receptor binding, identified 21 proteins, one of which was the predominantly ER resident chaperone protein GRP78. The interaction of GRP78 and ZIKV E was confirmed by co-immunoprecipitation and reciprocal co-immunoprecipitation, and indirect immunofluorescence staining showed intracellular and extracellular co-localization between GRP78 and ZIKV E. Antibodies directed against the N-terminus of GRP78 were able to inhibit ZIKV entry to host cells, resulting in significant reductions in the levels of ZIKV infection and viral production. Consistently, these reductions were also observed after down-regulation of GRP78 by siRNA. These results indicate that GRP78 can play a role mediating ZIKV binding, internalizat

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Author

Khongwichit, Sarawut (author);Sornjai, Wannapa (author);Jitobaom, Kunlakanya (author);Greenwood, Mingkwan (author);Greenwood, Michael P. (author);Hitakarun, Atitaya (author);Wikan, Nitwara (author);Murphy, David (author);Smith, Duncan R. (author)

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)