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Molecular characterization, biofilm analysis and experimental biofouling study of Fusarium isolates from recent cases of fungal keratitis in New York State by Madhu Dyavaiah; Rama Ramani; David S Chu; David C Ritterband; Mahendra K Shah; William A Samsonoff; Sudha Chaturvedi; Vishnu Chaturvedi is a Medicine article available to read on EtoBox.
What is Molecular characterization, biofilm analysis and experimental biofouling study of Fusarium isolates from recent cases of fungal keratitis in New York State about?
## Background To characterize __Fusarium__ isolates from recent cases of fungal keratitis in contact lens wearers, and to investigate fungal association with MoistureLoc solution. ## Methods We studied six fungal isolates from recent cases of keratitis in New York State. The isolates were characterized by nucleotide sequencing and phylogenetic analyses of multiple genes, and then typed using minisatellite and microsatellite probes. Experimental fungal biofilm formation was tested by standard methods. MoistureLoc solutions were tested in biofouling studies for their efficacy in elimination of __Fusarium__ contamination. ## Results __Fusarium solani__ – corneal ulcers (2 isolates), lens case (1 isolate), and __F. oxysporum__ – corneal ulcer (1 isolate), eye (1 isolate), were recovered from five patients. An opened bottle of MoistureLoc solution provided by a patient also yielded __F. solani__. Two distinct genotypes of __F. solani__ as well as of __F. oxysporum__ were present in the isolated strains. Remarkably, __F. solani__ strains from the lens case and lens solution in one instance were similar, based on phylogenetic analyses and molecular typing. The solution isolate of __F. sol
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- Author
- Madhu Dyavaiah; Rama Ramani; David S Chu; David C Ritterband; Mahendra K Shah; William A Samsonoff; Sudha Chaturvedi; Vishnu Chaturvedi
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2415)
- Published
- 2007
- Language
- EN
- Field
- Medicine (Health Sciences)