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Suppression of transforming growth factor-beta isoforms, TGF-β receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrix by Scheffer C.G. Tseng; De-Quan Li; Xiang Ma is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Down-regulation of the transforming growth factor-beta (TGF-␤) signaling system is a strategy for preventing scarring during wound healing. Human corneal and limbal fibroblasts were cultured on the stromal matrix side of preserved human amniotic membrane. The levels of TGF-␤1, ␤2, and ␤3 and TGF-␤ type II receptor transcripts and TGF-␤1 and ␤2 proteins were suppressed as early as 8 hr and more dramatically at 24 hr after contact with an amniotic membrane. This suppressive effect was accompanied by down-regulation of ␣-smooth muscle actin, EDA spliced form of fibronectin, and integrin ␣5. It persisted even when challenged by 10 ng/ml TGF-␤1. In contrast with their counterparts grown on plastic or in collagen gel, such suppression in amniotic membrane cultures remained complete after 1 week of culturing. Cells cultured on amniotic membrane showed significantly reduced [ 3 H]-thymidine incorporation compared to cells cultured on plastic and displayed no DNA fragmentation. These results reveal a novel mechanism by which the TGF-␤ signaling system, DNA synthesis, and subsequent myofibroblast differentiation can be suppressed by an amnionic membrane matrix. This action explains in part t

Who reads Suppression of transforming growth factor-beta isoforms, TGF-β receptor type II, and myofibroblast differentiation in cultured human corneal and limbal fibroblasts by amniotic membrane matrix?

Author

Scheffer C.G. Tseng; De-Quan Li; Xiang Ma

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)