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Antioxidant Activity and Role of Culture Condition in the Optimization of Red Pigment Production by Talaromyces purpureogenus KKP Through Response Surface Methodology by Keekan, Kishor Kumar; Hallur, Sweta; Modi, Prashant Kumar; Shastry, Rajesh P. is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The red pigment production by Talaromyces purpureogenus KKP, a soil isolate, was optimized by response surface methodology (RSM) in the present study. The cultural parameters, such as pH, temperature, dextrose, and peptone concentrations, were optimized for red pigment production using the central composite design (CCD) experimental design. A second-order quadratic model was used to calculate the relationships between the values at different levels of response. The optimum values of the selected variables under coded factors are 6.0, 27 °C, 2.25%, and 1.10% for pH, temperature, dextrose, and peptone, respectively. The selected variables were most effective in the enhancement of red pigment production at optimized culture conditions. In addition to optimization, the antioxidant activity of the pigment isolated in the present study was found to be promising with IC value (40 μg/ml). The HRMS data revealed the identification of delphinidin, limonene, 6-hydroxymethyl-7,8-dihydropterin, D-mannose 6-phosphate, and CDP-DG (18:0/18:0). The results of the present investigation will be added to the existing literature of red pigment production and its optimization by T. purpureogenus.

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Author

Keekan, Kishor Kumar; Hallur, Sweta; Modi, Prashant Kumar; Shastry, Rajesh P.

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Springer Nature (ISSN 0343-8651)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)