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Can I read Intra-islet GLP-1, but not CCK, is necessary for Î2-cell function in mouse and human islets on EtoBox?
Intra-islet GLP-1, but not CCK, is necessary for Î2-cell function in mouse and human islets by de Souza, Arnaldo Henrique; Tang, Jiayin; Yadev, Amanjot Kaur; Saghafi, Samuel T.; Kibbe, Carly R.; Linnemann, Amelia K.; Merrins, Matthew J.; Davis, Dawn Belt is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Intra-islet GLP-1, but not CCK, is necessary for Î2-cell function in mouse and human islets about?
Glucagon-like peptide 1 (GLP-1) and cholecystokinin (CCK) are gut-derived peptide hormones known to play important roles in the regulation of gastrointestinal motility and secretion, appetite, and food intake. We have previously demonstrated that both GLP-1 and CCK are produced in the endocrine pancreas of obese mice. Interestingly, while GLP-1 is well known to stimulate insulin secretion by the pancreatic β-cells, direct evidence of CCK promoting insulin release in human islets remains to be determined. Here, we tested whether islet-derived GLP-1 or CCK is necessary for the full stimulation of insulin secretion. We confirm that mouse pancreatic islets secrete GLP-1 and CCK, but only GLP-1 acts locally within the islet to promote insulin release __ex vivo__. GLP-1 is exclusively produced in approximately 50% of α-cells in lean mouse islets and 70% of α-cells in human islets, suggesting a paracrine α to β-cell signaling through the β-cell GLP-1 receptor. Additionally, we provide evidence that islet CCK expression is regulated by glucose, but its receptor signaling is not required during glucose-stimulated insulin secretion (GSIS). We also see no increase in GSIS in response to CCK p
Who reads Intra-islet GLP-1, but not CCK, is necessary for Î2-cell function in mouse and human islets?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- de Souza, Arnaldo Henrique; Tang, Jiayin; Yadev, Amanjot Kaur; Saghafi, Samuel T.; Kibbe, Carly R.; Linnemann, Amelia K.; Merrins, Matthew J.; Davis, Dawn Belt
- Publisher
- Springer Science and Business Media LLC; London: Nature Publishing Group; [London]: Springer Nature; Nature Publishing Group; Society for Mining, Metallurgy and Exploration Inc.; Research Square; Springer Nature; Island Press (ISSN 2045-2322)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)