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RFLP analysis of mitochondrial DNA from cytoplasmic male-sterile lines of pearl millet by R. Rajeshwari; S. Sivaramakrishnan; R. L. Smith; N. C. Subrahmanyam is a Agricultural and Biological Sciences article available to read on EtoBox.

What is RFLP analysis of mitochondrial DNA from cytoplasmic male-sterile lines of pearl millet about?

Mitochondrial DNA (mtDNA) from 13 cytoplasmic male-sterile (cms) lines from diverse sources were characterized by Southern blot hybridization to pearl millet and maize mtDNA probes. Hybridization patterns of mtDNA digested with PstI, BamHI, SmaI or XhoI and probed with 13.6-, 10.9-, 9.7- or 4.7-kb pearl millet mtDNA clones revealed similarities among the cms lines 5141 A and ICMA 1 (classified as the S-A1 type of cytoplasm based on fertility restoration patterns), PMC 30A and ICMA 2. The remaining cms lines formed a distinct group, within which three subgroups were evident. Among the maize mitochondiral gene clones used, the coxI probe revealed two distinct groups of cytoplasms similar to the pearl millet mtDNA clones. The atp9 probe differentiated the cms line 81 A4, derived from P. glaucum subsp. monodii, while the coxII gene probe did not detect any polymorphism among the cms lines studied. MtDNA digested with BamHI, PstI or XhoI and hybridized to the atp6 probe revealed distinct differences among the cms lines. The maize atp6 gene clone identified four distinct cytoplasmic groups and four subgroups within a main group. The mtDNA fragments hybridized to the atp6 gene probe with

Who reads RFLP analysis of mitochondrial DNA from cytoplasmic male-sterile lines of pearl millet?

Author

R. Rajeshwari; S. Sivaramakrishnan; R. L. Smith; N. C. Subrahmanyam

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc.; Springer Nature (ISSN 0040-5752)

Published

1994

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)