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Can I read Loss of Endogenously Cycling Adult Cardiomyocytes Worsens Myocardial Function on EtoBox?
Loss of Endogenously Cycling Adult Cardiomyocytes Worsens Myocardial Function by Bradley, Leigh A; Young, Alexander; Li, Hongbin; Billcheck, Helen O; Wolf, Matthew J is a Medicine article available to read on EtoBox.
What is Loss of Endogenously Cycling Adult Cardiomyocytes Worsens Myocardial Function about?
## Rationale: Endogenously cycling adult cardiomyocytes increase after myocardial infarction (MI) but remain scarce and are generally thought not to contribute to myocardial function. However, this broadly held assumption has not been tested, mainly because of the lack of transgenic reporters that restrict Cre expression to adult cardiomyocytes that reenter the cell cycle. ## Objective: We created and validated a new transgenic mouse, αMHC (alpha myosin heavy chain) -MerDreMer-Ki67p-RoxedCre (denoted αDKRC [cardiomyocyte-specific αMHC-MerDreMer-Ki67p-RoxedCre]) that restricts Cre expression to cycling adult cardiomyocytes and uniquely integrates spatial and temporal adult cardiomyocyte cycling events based on the DNA specificities of orthologous Dre and Cre recombinases. We then created αDKRC::DTA mice that expressed an inducible diphtheria toxin in adult cycling cardiomyocytes and examined the effects of ablating these endogenously cycling cardiomyocytes on myocardial function after ischemic-reperfusion (I/R) MI. ## Methods and Results: A tandem αDKRC transgene was designed, validated in cultured cells, and used to make transgenic mice. The αDKRC transgene integrated between MYH6
Who reads Loss of Endogenously Cycling Adult Cardiomyocytes Worsens Myocardial Function?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Bradley, Leigh A; Young, Alexander; Li, Hongbin; Billcheck, Helen O; Wolf, Matthew J
- Publisher
- Lippincott Williams and Wilkins; Ovid Technologies Wolters Kluwer -American Heart Association; Lippincott Williams & Wilkins Ltd.; Ovid Technologies (Wolters Kluwer Health) (ISSN 0009-7330)
- Published
- 2020
- Language
- EN
- Field
- Medicine (Health Sciences)