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[17] Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis by Michael W. Hunkapiller; Eva Lujan; Frank Ostrander; Leroy E. Hood is a book available to read on EtoBox.

What is [17] Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysis about?

Polyacrylamide gels are one of the most widely used media for the analytical separation of proteins and peptides. Electrophoretic separations based upon intrinsic charge-to-mass ratio, 1 apparent molecular weight in the presence of sodium dodecyl sulfate (SDS), 2 and isoelectric focusing 3 each possess remarkable resolving power using amounts of proteins ranging from a few milligrams to fractions of micrograms. When two of these techniques, molecular sieving with SDS and isoelectric focusing with ampholytes, are combined in two-dimensional separations, 4 complex mixtures can be resolved into as many as several thousand components in a single experiment. Although these procedures are generally thought of as analytical techniques, they also provide a powerful preparative technique for small quantities of protein if the protein can be removed from the gel once the resolving electrophoresis is accomplished and the protein positions are located. If the proteins have not been fixed in the gel by exposure to acidic conditions during staining with dyes, some proteins can be eluted from the gels by soaking crushed gel pieces in dilute SDS solutions. However, low recoveries, contamination wi

Author

Michael W. Hunkapiller; Eva Lujan; Frank Ostrander; Leroy E. Hood

Published

1983

Language

EN