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Untargeted Mass Spectrometry-based Metabolomics Approach Unveils Biochemical Changes in Compound Probiotic Fermented Milk During Fermentation by Yaru Sun; Shuai Guo; Ting Wu; Jingwen Zhang; Lai-Yu Kwok; Zhihong Sun; Heping Zhang; Jicheng Wang is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Untargeted Mass Spectrometry-based Metabolomics Approach Unveils Biochemical Changes in Compound Probiotic Fermented Milk During Fermentation about?
Probiotic functional products have drawn wide attention because of their increasing popularity. However, few studies have analyzed probiotic-specific metabolism in the fermentation process. This study applied UPLC-QE-MS-based metabolomics to track changes in the milk metabolomes in the course of fermentation by two probiotic strains, __Lacticaseibacillus paracasei__ PC-01 and __Bifidobacterium adolescentis__ B8589. We observed substantial changes in the probiotic fermented milk metabolome between 0 and 36 h of fermentation, and the differences between the milk metabolomes at the interim period (36 h and 60 h) and the ripening stage (60 h and 72 h) were less obvious. A number of time point-specific differential metabolites were identified, mainly belonging to organic acids, amino acids, and fatty acids. Nine of the identified differential metabolites are linked to the tricarboxylic acid cycle, glutamate metabolism, and fatty acid metabolism. The contents of pyruvic acid, γ-aminobutyric acid, and capric acid increased at the end of fermentation, which can contribute to the nutritional quality and functional properties of the probiotic fermented milk. This time-course metabolomics stu
Who reads Untargeted Mass Spectrometry-based Metabolomics Approach Unveils Biochemical Changes in Compound Probiotic Fermented Milk During Fermentation?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Yaru Sun; Shuai Guo; Ting Wu; Jingwen Zhang; Lai-Yu Kwok; Zhihong Sun; Heping Zhang; Jicheng Wang
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)