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Effects of aging on inositol 1,4,5-triphosphate-induced Ca2+ release in unfertilized mouse oocytes by Toshifumi Takahashi; Hidekazu Saito; Masahiko Hiroi; Katsuhiko Doi; Eiji Takahashi is a Medicine article available to read on EtoBox.

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We previously demonstrated in the mouse oocyte that in vivo postovulatory aging significantly suppresses activity of the endoplasmic reticulum (ER) Ca 2ϩ -ATPase (Igarashi et al. 1997. Mol Reprod Dev 48:383-390). We undertook the present study to further examine the effects of oocyte aging on Ca 2ϩ release from the inositol 1,4,5-triphosphate (InsP 3 )sensitive Ca 2ϩ channels of the ER membrane, because not only Ca 2ϩ reuptake, but also Ca 2ϩ release from the ER, substantially affect Ca 2ϩ oscillations in fertilized oocytes. A transient increase in cytosolic free Ca 2ϩ concentration ([Ca 2ϩ ] i ) was induced by photolysis of caged InsP 3 microinjected into the cytoplasm in both fresh (14 hr post hCG) and aged (20 hr or 24 hr post hCG) oocytes, where the maximum rate of increase in [Ca 2ϩ ] i significantly decreased in the aged oocytes. Reduced ER Ca 2ϩ release in the aged oocyte may not be attributable to aging-related desensitization of the InsP 3sensitive Ca 2ϩ channels in the ER because concentrations of caged InsP 3 for half maximal [Ca 2ϩ ] i increase were identical for fresh and aged oocytes. The peak [Ca 2ϩ ] i response following administration of 5 M thapsigargin, a specifi

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Author

Toshifumi Takahashi; Hidekazu Saito; Masahiko Hiroi; Katsuhiko Doi; Eiji Takahashi

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1040-452X)

Published

2000

Language

EN

Field

Medicine (Life Sciences)