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Metabolite and isotopomer balancing in the analysis of metabolic cycles: I. Theory by Maria I. Klapa; Sung M. Park; Anthony J. Sinskey; Gregory Stephanopoulos is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Proper analysis of label distribution in metabolic pathway intermediates is critical for correct interpretation of experimental data and strategic experimental design. While, for example, 13 C nuclear magnetic resonance (NMR) spectroscopy is usually limited to the measurement of degrees of 13 C enrichment, more information about metabolic fluxes can be extracted from the fine structure of NMR spectra, or molecular weight distributions of isotopomers of metabolic intermediates (measured by gas chromatography-mass spectrometry). For this purpose, rigorous accounting for the contribution of all pathways to label distribution is required, especially contributions resulting from multiple turns of metabolic cycles. In this paper we present a mathematical model developed to analyze isotopomer distributions of tricarboxylic acid cycle (TCA) intermediates following the administration of 13 C (or 14 C) labeled substrates. The theory presented provides the basis to analyze 13 C NMR spectra and molecular weight distributions of metabolites. In a companion paper (Park et al., 1999), the theory is applied to the analysis of several cases of biological significance.

Who reads Metabolite and isotopomer balancing in the analysis of metabolic cycles: I. Theory?

Author

Maria I. Klapa; Sung M. Park; Anthony J. Sinskey; Gregory Stephanopoulos

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

1999

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)