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Automated analysis of NeuronJ tracing data by Jonathan Popko; Adelaide Fernandes; Dora Brites; Lorene M. Lanier is a scholarly article available to read on EtoBox.

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## Abstract Studies of neuronal differentiation __in vitro__ often involve tracing and analysis of neurites. NeuronJ (Meijering et al., Cytometry Part A 2004;58A:167–176; http://www.imagescience.org/meijering/software/neuronj/) is a program that can be used for semiautomated tracing of individual neurons; when tracing is completed, a text file containing neurite length measurements is generated. Using cultured hippocampal neurons, we have found that to reach statistical significance it is generally necessary to trace about 100 neurons in each treatment group. Posttracing data analysis requires importing each text file into a statistics program. Analysis of distinct parameters, such as effects of a treatment on axonal versus dendritic branching, requires a great deal of time consuming posttracing data manipulation. We have developed XL\_Calculations, a Java‐based program that performs batch analysis on NeuronJ measurement files and automatically makes multiple calculations, including the number, length, and total output (sum length) of primary, secondary, and tertiary neurites on axons and dendrites, and writes the calculations into an Excel worksheet. Batch processing of NeuronJ me

Author

Jonathan Popko; Adelaide Fernandes; Dora Brites; Lorene M. Lanier

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; John Wiley & Sons Ltd.; Wiley (ISSN 0196-4763)

Published

2008

Language

EN