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Characterization of a tobacco epoxide hydrolase gene induced during the resistance response to TMV by Ailan Guo; Jörg Durner; Daniel F. Klessig is a scholarly article available to read on EtoBox.

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## Summary A clone encoding a putative soluble epoxide hydrolase (EH‐1), an enzyme which converts epoxides to diols, was isolated by differential screening of a cDNA library prepared from tobacco mosaic virus (TMV)‐infected tobacco leaves. To confirm that __EH‐1__ encodes an epoxide hydrolase, the recombinant EH‐1 protein produced in bacteria was shown to have high epoxide hydrolase activity __in vitro__. Infection of resistant but not susceptible tobacco cultivars induced the accumulation of __EH‐1__ transcripts in both the inoculated and uninoculated, systemic leaves. __EH‐1__ expression was also induced in the inoculated and systemic tissues of TMV‐infected NahG plants, which are unable to accumulate salicylic acid (SA). However, __EH‐1__ expression in the inoculated leaves of NahG plants was delayed, whilst in the systemic leaves the induction was both later and weaker, compared to that observed in wild‐type plants. Furthermore, exogenously applied SA or its functional analog 2,6‐dichloroisonicotinic acid (INA) caused a rapid and transient accumulation of __EH‐1__ transcripts, whereas an inactive SA analog did not. Thus, the induction of __EH‐1__ gene expression appears to be r

Author

Ailan Guo; Jörg Durner; Daniel F. Klessig

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0960-7412)

Published

1998

Language

EN