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Ratiometric Fluorogenic RNA-Based Sensors for Imaging Live-Cell Dynamics of Small Molecules by Wu, Rigumula; Karunanayake Mudiyanselage, Aruni P.K.K.; Ren, Kewei; Sun, Zhining; Tian, Qian; Zhao, Bin; Bagheri, Yousef; Lutati, David; Keshri, Puspam; You, Mingxu is a Materials Science article available to read on EtoBox.

What is Ratiometric Fluorogenic RNA-Based Sensors for Imaging Live-Cell Dynamics of Small Molecules about?

Imaging the cellular dynamics of metabolites and signaling molecules is critical for understanding various metabolism and signal transduction pathways. Genetically encoded RNA-based sensors are emerging powerful tools for this purpose. However, it was challenging to use these sensors to precisely determine the intracellular concentrations of target analytes. To solve this problem, we have recently developed ratiometric sensors using an orthogonal pair of RNA/fluorophore conjugates: Broccoli/DFHBI-1T (3,5-difluoro-4-hydroxybenzylidene-1-trifluoroethyl-imidazolinone) and DNB (dinitroaniline-binding aptamer)/SR-DN (sulforhodamine B-dinitroaniline). The cellular DNB-to-Broccoli fluorescence intensity ratio can be directly applied to quantify the target concentrations at the single-cell level. Unfortunately, due to the instability of the SR-DN dye, this ratiometric sensor is difficult to use for monitoring target dynamics. Herein, by replacing SR-DN with a stable TMR (tetramethylrhodamine)-DN dye, we developed a ratiometric sensor system based on Broccoli/DFHBI-1T and DNB/TMR-DN, which can be used for dynamic imaging in living cells. We believe these advanced genetically encoded ratiome

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Author

Wu, Rigumula; Karunanayake Mudiyanselage, Aruni P.K.K.; Ren, Kewei; Sun, Zhining; Tian, Qian; Zhao, Bin; Bagheri, Yousef; Lutati, David; Keshri, Puspam; You, Mingxu

Publisher

American Chemical Society; American Chemical Society (ACS) (ISSN 2576-6422)

Published

2020

Language

EN

Field

Materials Science (Physical Sciences)