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What is Carbohydrate Analysis: DNS Assay Guide about?

This document provides instructions for a lab exercise on carbohydrate analysis using the DNS assay. Students will create a standard curve using glucose standards of known concentrations to determine the concentration of an unknown sample. The DNS assay exploits the ability of reducing sugars like glucose to reduce 3,5-dinitrosalicylic acid to a colored product, which is measured spectrophotometrically at 540nm. Absorbance values will be plotted against concentration on a standard curve to determine the unk

Author

Trung Huynh

Language

EN