Can I read Simple sequence repeat (SSR) markers for Elymus , Pseudoroegneria and Pascopyrum species ( Triticeae : Gramineae ) on EtoBox?

Simple sequence repeat (SSR) markers for Elymus , Pseudoroegneria and Pascopyrum species ( Triticeae : Gramineae ) by I. W. Mott; S. R. Larson; B. S. Bushman is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Simple sequence repeat (SSR) markers for Elymus , Pseudoroegneria and Pascopyrum species ( Triticeae : Gramineae ) about?

The __Triticeae__ tribe (__Poaceae__) includes several important cereal crops, cultivated forages, annual and perennial grass weeds and ecologically diverse native North American grasses. __Elymus__ L. is the largest and most complex genus in the __Triticeae__ tribe with approximately 150 polyploid perennial grass species occurring worldwide. The genomic constitutions of approximately 40% of the __Elymus__ species are unknown. Molecular markers are needed to facilitate genetic analysis of diversity and functional traits in these species. We have developed simple sequence repeat (SSR) markers for use in __Elymus__ based on __Elymus__ expressed sequence tag sequences. To test the polymorphic content and transferability of these SSRs, 100 SSR primer pairs were tested on 84 plants representing seven North American __Elymus__, __Pseudoroegneria__ and __Pascopyrum__ species. The number of bands produced from each of the SSRs ranged from 1 to 11 with an average of 4.3 bands/SSR. A subset of the 23 most polymorphic SSRs produced 142 bands, an average of 6.2 bands/SSR. Binary data from the 100 SSRs successfully separated all individuals into their respective accessions in a neighbour-joinin

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Author

I. W. Mott; S. R. Larson; B. S. Bushman

Publisher

Cambridge University Press (CUP)

Published

2011

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)