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Remodeling of Junctional Complexes During the Development of the Outer Blood-retinal Barrier by Christopher D. Williams; Lawrence J. Rizzolo is a Medicine article available to read on EtoBox.

What is Remodeling of Junctional Complexes During the Development of the Outer Blood-retinal Barrier about?

Background: The retinal pigment epithelium (RPE) forms the outer blood-retinal barrier by separating the neural retina from fenestrated capillaries in the choroid. The barrier depends upon tight junctions within the apical junctional complexes that bind neighboring cells. During development, permeability decreases as the apical junctional complex gradually matures. To investigate this process, the composition of the apical junctional complex was monitored during RPE development in chicken embryos. Methods: Permeability was monitored by incubating freshly isolated RPE/choroid in medium containing horseradish peroxidase followed by histochemical staining and electron microscopy. The expression of the tight junction proteins, ZO-1 and occludin, was determined by immunofluorescence and immunoblotting. Development of the RPE apical junctional complex was to compared to the homologous complex that forms the outer limiting membrane of the neural retina. Results: The apical junctional complex of the RPE was permeable to horseradish peroxidase until embryonic day 10-12. Two putative forms of ZO-1 had approximately the same molecular mass as mammalian ZO-1 and were present in the apical junc

Who reads Remodeling of Junctional Complexes During the Development of the Outer Blood-retinal Barrier?

Author

Christopher D. Williams; Lawrence J. Rizzolo

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0003-276X)

Published

1998

Language

EN

Field

Medicine (Health Sciences)