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The Role of NLRP3 and IL-1Î2 in Refractory Epilepsy Brain Injury by Wu, Chunfeng; Zhang, Gang; Chen, Lei; Kim, Samuel; Yu, Jie; Hu, Guo; Chen, Jing; Huang, Yanjun; Zheng, Guo; Huang, Songming is a scholarly article available to read on EtoBox.

What is The Role of NLRP3 and IL-1Î2 in Refractory Epilepsy Brain Injury about?

**Objective:** The objective of this study was to investigate the roles and mechanisms of inflammatory mediators NLRP3 and IL-1β in refractory temporal epilepsy brain injury. **Method:** First, the brain tissue and the peripheral blood of children undergoing intractable temporal lobe epilepsy surgery were analyzed as research objects. The expression levels of NLRP3 in brain tissue and IL-1β in blood were measured. A model of temporal lobe epilepsy was established using wild-type and NLRP3 knockout 129 mice. Pilocarpine was injected intraperitoneally into the experimental group, and isovolumetric saline was injected intraperitoneally into the control group (__n__ = 8 in each group). The expression of IL-1β in the peripheral blood, cerebral cortex, and hippocampus of mice was measured by ELISA at 3 h, 24 h, 3 days, and 7 days after modeling. Fluoro-Jade B (FJB) and TUNEL methods were used to determine necrosis and apoptosis in hippocampal neurons, respectively, and the expression of NLRP3 in the cortex was measured by immunofluorescence methods. **Result:** (1) The IL-1β levels in the peripheral blood of children with intractable temporal lobe epilepsy were higher than those in the c

Author

Wu, Chunfeng; Zhang, Gang; Chen, Lei; Kim, Samuel; Yu, Jie; Hu, Guo; Chen, Jing; Huang, Yanjun; Zheng, Guo; Huang, Songming

Publisher

Frontiers; Frontiers Media SA; Frontiers Media S.A.; Lausanne: Frontiers Media S.A., 2010- (ISSN 1664-2295)

Published

2020

Language

EN