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Ultrasensitive and Low-Cost Paper-Based Graphene Oxide Nanobiosensor for Monitoring Water-Borne Bacterial Contamination by Stalin Karuppiah; Narayan Chandra Mishra; Wei-Chen Tsai; Wei-Ssu Liao; Chia-Fu Chou is a scholarly article available to read on EtoBox.

What is Ultrasensitive and Low-Cost Paper-Based Graphene Oxide Nanobiosensor for Monitoring Water-Borne Bacterial Contamination about?

Water-borne pathogens are mostly generated due to poor sanitation, industrial effluents, and sewage sludge, leading to a significant increase in mortality rate. To prevent this, we need a simple, user-friendly, and rapid on-site detection tool of pathogens, i.e., a biosensor. As contaminated water mainly contains (80%) coliform bacteria, of which is the major species, we have developed a screen-printed paper-based, label-free biosensor for the detection of in water. A nanoarchitectured graphene oxide (GO), as a fast electron-transfer flatland, was deposited on the screen-printed graphene (G) on a hydrophobic paper, followed by the immobilization of lectin Concanavalin A (ConA) as a biorecognition element for a GGO\_ConA-biosensing electrode. The electrochemical characterization of GGO\_ConA shows fast electron transfer with a calculated electroactive surface area of 0.16 cm. The biosensor performance was tested in the sludge water and beach water (real sample) as an analyte using the electrochemical impedance spectroscopy (EIS) technique. The charge-transfer resistance () of GGO\_ConA increases linearly with the bacterial concentration in the range of 10-10 CFU mL with an estimated

Author

Stalin Karuppiah; Narayan Chandra Mishra; Wei-Chen Tsai; Wei-Ssu Liao; Chia-Fu Chou

Publisher

American Chemical Society (ACS)

Published

2021

Language

EN