Can I read Isocitrate dehydrogenases from liver of Squalus acanthias (spiny dogfish) and citrate formation by isolated mitochondria on EtoBox?

Isocitrate dehydrogenases from liver of Squalus acanthias (spiny dogfish) and citrate formation by isolated mitochondria by Kristine M. Saxrud; David O. Lambeth; Paul M. Anderson is a Environmental Science article available to read on EtoBox.

What is Isocitrate dehydrogenases from liver of Squalus acanthias (spiny dogfish) and citrate formation by isolated mitochondria about?

Isolated mitochondria from liver of Squalus acanthias (spiny dogfish) catalyze formation of citrate from a-ketoglutarate at a rate corresponding to >0.03 pmoles per min per g tissue. Malate alone does not support citrate formation, but stimulates citrate formation from aketoglutarate = threefold. These reactions appear to proceed by reductive carboxylation catalyzed by the mitochondrial NADP-dependent isocitrate dehydrogenase, i.e., reversal of the Krebs cycle from a-ketoglutarate to citrate. The NADP-dependent isocitrate dehydrogenases present in the mitochondria and cytosol were isolated and shown to have different N-terminal amino acid sequences, indicating that they are encoded by different genes. The kinetic and structural properties of the mitochondrial enzyme were found to be similar to that of NADP-dependent isocitrate dehydrogenases from other species. NAD-dependent isocitrate dehydrogenase activity could not be detected in liver extracts or in mitochondrial or cytosolic subfractions of liver extracts. The level of activity of the reverse reaction (~0.4 pmoles per min per g liver), i.e., reductive carboxylation, of the mitochondrial isocitrate dehydrogenase is sufficient t

Who reads Isocitrate dehydrogenases from liver of Squalus acanthias (spiny dogfish) and citrate formation by isolated mitochondria?

Author

Kristine M. Saxrud; David O. Lambeth; Paul M. Anderson

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-104X)

Published

1996

Language

EN

Field

Environmental Science (Life Sciences)