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Can I read Genetic and biochemical characterization of a short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus on EtoBox?
Genetic and biochemical characterization of a short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus by John van der Oost; Wilfried G. B. Voorhorst; Servé W. M. Kengen; Ans C. M. Geerling; Vincent Wittenhorst; Yannick Gueguen; Willem M. de Vos is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Genetic and biochemical characterization of a short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus about?
The gene encoding a short‐chain alcohol dehydrogenase, AdhA, has been identified in the hyperthermophilic archaeon __Pyrococcus furiosus__, as part of an operon that encodes two glycosyl hydrolases, the β‐glucosidase CelB and the endoglucanase LamA. The __adhA__ gene was functionally expressed in __Escherichia coli, and__ AdhA was subsequently purified to homogeneity. The quaternary structure of AdhA is a dimer of identical 26‐kDa subunits. AdhA is an NADPH‐dependent oxidoreductase that converts alcohols to the corresponding aldehydes/ketones and vice versa, with a rather broad substrate specificity. Maximal specific activities were observed with 2‐pentanol (46 U·mg^−1^) and pyruvaldehyde (32 U·mg^−1^) in the oxidative and reductive reaction, respectively. AdhA has an optimal activity at 90 °C, at which temperature it has a half life of 22.5 h. The expression of the __adhA__ gene in __P. furiosus__ was demonstrated by activity measurements and immunoblot analysis of cell extracts. A role of this novel type of archaeal alcohol dehydrogenase in carbohydrate fermentation is discussed.
Who reads Genetic and biochemical characterization of a short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- John van der Oost; Wilfried G. B. Voorhorst; Servé W. M. Kengen; Ans C. M. Geerling; Vincent Wittenhorst; Yannick Gueguen; Willem M. de Vos
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2001
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Physical Sciences)