About this document
2005 JCH GSKB Maldi Bmcbiochem2005 by Jeffrey Howard is a document available to read on EtoBox.
This article presents a novel mass spectrometry-based assay for measuring Glycogen Synthase Kinase-3beta (GSK-3β) activity, addressing the limitations of traditional radioactive kinase assays. The developed method utilizes synthetic peptide substrates and surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) to detect phosphorylation events sensitively and accurately. The assay shows potential for high-throughput screening and understanding GSK-3β
- Author
- Jeffrey Howard
- Language
- EN